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bca protein array kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bca protein array kit
    Bca Protein Array Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bca+protein+array+kit/BCA+Protein+Assay+Kit/pm41390720-163-24-28
    Average 99 stars, based on 1 article reviews
    bca protein array kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Homogenization:

    Article Title: Targeted deletion of EMMPRIN in microglia/macrophages mitigates neuronal death in intracerebral hemorrhage.
    Article Snippet: .. Total protein was extracted by homogenization in lysis buffer (2% SDS, 200mM ammonium bicarbonate, protease inhibitor tablets, 0.1 mM EDTA) and quantified using the BCA Protein Array Kit (Pierce, Rockford, IL, USA). ..

    Article Title: Targeted deletion of EMMPRIN in microglia/macrophages mitigates neuronal death in intracerebral hemorrhage
    Article Snippet: .. Total protein was extracted by homogenization in lysis buffer (2% SDS, 200mM ammonium bicarbonate, protease inhibitor tablets, 0.1 mM EDTA) and quantified using the BCA Protein Array Kit (Pierce, Rockford, IL, USA). ..

    Lysis:

    Article Title: Targeted deletion of EMMPRIN in microglia/macrophages mitigates neuronal death in intracerebral hemorrhage.
    Article Snippet: .. Total protein was extracted by homogenization in lysis buffer (2% SDS, 200mM ammonium bicarbonate, protease inhibitor tablets, 0.1 mM EDTA) and quantified using the BCA Protein Array Kit (Pierce, Rockford, IL, USA). ..

    Article Title: Targeted deletion of EMMPRIN in microglia/macrophages mitigates neuronal death in intracerebral hemorrhage
    Article Snippet: .. Total protein was extracted by homogenization in lysis buffer (2% SDS, 200mM ammonium bicarbonate, protease inhibitor tablets, 0.1 mM EDTA) and quantified using the BCA Protein Array Kit (Pierce, Rockford, IL, USA). ..

    Protease Inhibitor:

    Article Title: Targeted deletion of EMMPRIN in microglia/macrophages mitigates neuronal death in intracerebral hemorrhage.
    Article Snippet: .. Total protein was extracted by homogenization in lysis buffer (2% SDS, 200mM ammonium bicarbonate, protease inhibitor tablets, 0.1 mM EDTA) and quantified using the BCA Protein Array Kit (Pierce, Rockford, IL, USA). ..

    Article Title: Targeted deletion of EMMPRIN in microglia/macrophages mitigates neuronal death in intracerebral hemorrhage
    Article Snippet: .. Total protein was extracted by homogenization in lysis buffer (2% SDS, 200mM ammonium bicarbonate, protease inhibitor tablets, 0.1 mM EDTA) and quantified using the BCA Protein Array Kit (Pierce, Rockford, IL, USA). ..

    Protein Array:

    Article Title: Targeted deletion of EMMPRIN in microglia/macrophages mitigates neuronal death in intracerebral hemorrhage.
    Article Snippet: .. Total protein was extracted by homogenization in lysis buffer (2% SDS, 200mM ammonium bicarbonate, protease inhibitor tablets, 0.1 mM EDTA) and quantified using the BCA Protein Array Kit (Pierce, Rockford, IL, USA). ..

    Article Title: Targeted deletion of EMMPRIN in microglia/macrophages mitigates neuronal death in intracerebral hemorrhage
    Article Snippet: .. Total protein was extracted by homogenization in lysis buffer (2% SDS, 200mM ammonium bicarbonate, protease inhibitor tablets, 0.1 mM EDTA) and quantified using the BCA Protein Array Kit (Pierce, Rockford, IL, USA). ..

    Article Title: Adiponectin-mediated promotion of CD44 suppresses diabetic vascular inflammatory effects
    Article Snippet: Antibodies against APPL1 (#3858), Histone 2A (#7631), GAPDH (#5174), β-catenin(#8480), Non-phospho (Active) β-catenin(#8814), Phospho-β-catenin(#9561), Reptin(#12668) were from Cell Signaling Technology (Beverly, MA). . Small interfering RNA transfection RNA oligonucleotides complementary to Occludin, APPL1 target sequences silenced respective gene expression. .. Protein concentrations were determined by BCA Protein Array Kit (Thermo Fisher Scientific, Inc. 23227). ..

    Article Title: Hyperoside mitigates PCOS-associated adipogenesis and insulin resistance by regulating NCOA2-mediated PPAR-γ ubiquitination and degradation.
    Article Snippet: Aims: Polycystic ovary syndrome (PCOS) is closely associated with metabolic disorders such as insulin resistance and obesity, but the role of adipogenesis in its pathophysiology remains unclear.. This study investigates the role of adipogenesis in PCOS development and evaluates whether hyperoside (HPS), an anti-adipogenic herbal compound, can improve PCOS by inhibiting adipogenesis.. Main methods: A combination of in vivo and in vitro models was used to assess the impact of HPS on ovarian function, insulin resistance, and adipogenesis.

    Article Title: Cigarette smoking induces lung cancer tumorigenesis via upregulation of the WNT/β-catenin signaling pathway.
    Article Snippet: Lung cancer has the highest mortality rate compared to any other cancer worldwide, and cigarette smoking is one of the major etiological factors.. How cigarette smoke (CS) induces tumorigenesis in healthy cells is still not completely understood.. In this study, we treated healthy human bronchial epithelial cells (16HBE14o) with 1 % cigarette smoke extract (CSE) for one week.

    Concentration Assay:

    Article Title: Hyperoside mitigates PCOS-associated adipogenesis and insulin resistance by regulating NCOA2-mediated PPAR-γ ubiquitination and degradation.
    Article Snippet: Aims: Polycystic ovary syndrome (PCOS) is closely associated with metabolic disorders such as insulin resistance and obesity, but the role of adipogenesis in its pathophysiology remains unclear.. This study investigates the role of adipogenesis in PCOS development and evaluates whether hyperoside (HPS), an anti-adipogenic herbal compound, can improve PCOS by inhibiting adipogenesis.. Main methods: A combination of in vivo and in vitro models was used to assess the impact of HPS on ovarian function, insulin resistance, and adipogenesis.

    Protein Concentration:

    Article Title: Cigarette smoking induces lung cancer tumorigenesis via upregulation of the WNT/β-catenin signaling pathway.
    Article Snippet: Lung cancer has the highest mortality rate compared to any other cancer worldwide, and cigarette smoking is one of the major etiological factors.. How cigarette smoke (CS) induces tumorigenesis in healthy cells is still not completely understood.. In this study, we treated healthy human bronchial epithelial cells (16HBE14o) with 1 % cigarette smoke extract (CSE) for one week.

    Reverse Transcription:

    Article Title: Hyperoside mitigates PCOS-associated adipogenesis and insulin resistance by regulating NCOA2-mediated PPAR-γ ubiquitination and degradation.
    Article Snippet: Aims: Polycystic ovary syndrome (PCOS) is closely associated with metabolic disorders such as insulin resistance and obesity, but the role of adipogenesis in its pathophysiology remains unclear.. This study investigates the role of adipogenesis in PCOS development and evaluates whether hyperoside (HPS), an anti-adipogenic herbal compound, can improve PCOS by inhibiting adipogenesis.. Main methods: A combination of in vivo and in vitro models was used to assess the impact of HPS on ovarian function, insulin resistance, and adipogenesis.

    SYBR Green Assay:

    Article Title: Hyperoside mitigates PCOS-associated adipogenesis and insulin resistance by regulating NCOA2-mediated PPAR-γ ubiquitination and degradation.
    Article Snippet: Aims: Polycystic ovary syndrome (PCOS) is closely associated with metabolic disorders such as insulin resistance and obesity, but the role of adipogenesis in its pathophysiology remains unclear.. This study investigates the role of adipogenesis in PCOS development and evaluates whether hyperoside (HPS), an anti-adipogenic herbal compound, can improve PCOS by inhibiting adipogenesis.. Main methods: A combination of in vivo and in vitro models was used to assess the impact of HPS on ovarian function, insulin resistance, and adipogenesis.

    other:

    Article Title: Adiponectin-mediated promotion of CD44 suppresses diabetic vascular inflammatory effects
    Article Snippet: Incubate at 4°C for 30 min. Cleared lysate was then incubated with normal immunoglobulin G (IgG) and anti-reptin (Cell Signaling Technology, #12668)) primary antibodies together with 15 μL pre-washed Protein A beads at 4 °C overnight.



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    Key resources table
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    Key resources table

    Journal: iScience

    Article Title: Adiponectin-mediated promotion of CD44 suppresses diabetic vascular inflammatory effects

    doi: 10.1016/j.isci.2023.106428

    Figure Lengend Snippet: Key resources table

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit monoclonal anti-reptin Cell Signaling Technology Cat#12668S; RRID: AB_2797987 Rabbit monoclonal anti-IgG Cell Signaling Technology Cat#14708S; RRID: AB_2798581 Rabbit monoclonal anti-APPL1 Cell Signaling Technology Cat#3858S; RRID: AB_2056989 Rabbit monoclonal anti-Histone 2A Cell Signaling Technology Cat#7631S; RRID: AB_10860771 Rabbit monoclonal anti-GAPDH Cell Signaling Technology Cat#5174S; RRID: AB_10622025 Rabbit monoclonal anti-β-catenin Cell Signaling Technology Cat#8480S; RRID: AB_11127855 Rabbit monoclonal anti-Non-β-catenin Cell Signaling Technology Cat#8814S; RRID: AB_11127203 NF-κB Pathway Antibody Sampler Kit Cell Signaling Technology Cat#9936T; RRID: AB_561197 Rabbit monoclonal anti-CD44 Cell Signaling Technology Cat#37259S; RRID: AB_2750879 Rabbit monoclonal anti-ICAM-1 Cell Signaling Technology Cat#67836S; RRID: AB_2799738 Biological samples Serum of Healthy control subjects and Diabetes patients Anzhen Hospital, Capital Medical University, Beijing, China https://anzhen.org/ Chemicals, peptides, and recombinant proteins Recombinant Human gAcrp30/Adipolean Peprotech.inc CAS:25-450-21 Recombinant CD44 protein ®Sangon Biotech CAS:D622619 Critical commercial assays BCA Protein Array Kit Thermo Fisher Scientific, Inc. CAS:23227 Qproteome Cell Compartment Kit CAS:37502 The Transcription Factor Activation Profiling Plate Array II Signosis, Sunnyvale, CA CAS: FA-1002 RT2 ProfilerTM PCR Array Human Transcription Factors Qiagen, USA GeneGlobe ID-PAHS-075ZA-24; CAS: 330231 ELISA Kit for Adiponectin (ADPN) Cloud-Clone Corp CAS: SEA605Hu ELISA Kit for CD44 Cloud-Clone Corp CAS: SEA670Hu EZ-Link Sulfo-NHS-LC-Biotinylation kit Thermo Fisher Scientific, Inc. CAS:21435 Deposited data Raw and analyzed data This paper GEO: GSE217607 Experimental models: Cell lines Human umbilical vein endothelial cells (HUVECs): 4201HUM-CCTCC00635 NICR http://cellresource.cn/fdetail.aspx?id=5307/ Experimental models: Organisms/strains Mouse: APPL1 −/− , 8 weeks’s old BRL Medicine Inc. N/A Mouse: APN −/− , 8 weeks’s old Gift N/A Oligonucleotides siRNA targeting sequence: APPL1 #1: UCUCACCUGACUUCGAAACU This paper N/A siRNA targeting sequence: CD44 #1: GAACAAGGAGUCGUCAGAAACUCCA This paper N/A Primers, see Table S2 This paper N/A Software and algorithms GraphPad Prism 8.0 GraphPad Software Inc., San Diego, CA https://www.graphpad.com/ SPSS Statistics 25.0 SPSS Inc., Chicago, IL https://www.ibm.com/ Open in a separate window Key resources table .

    Techniques: Recombinant, Protein Array, Activation Assay, Enzyme-linked Immunosorbent Assay, Sequencing, Software